Instrument: Illumina NovaSeq 6000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: Each CNS was isolated per mouse. A cell suspension was generated by papain digestion, neutralization, and 70um filtration of cell extract. Cells were centrifuged, labeled with Miltenyi anti-Dead Cell and anti-myelin kits, then magnetically purified and washed. Cells were sorted by flow cytometry based on mCherry fluorescence. Post-sort, cells were washed then processed by inDrop Libraries were created according to the InDrop v3 protocol derived from Klein et al. Cell 2015. inDrop scRNA-Seq